John Zullo's Summer Research 1998
John Zullo, BS in Physics December 1998
John is currently employed at Brookhaven National Laboratory.

M.U.S.C. Summer Research Project
I was awarded an internship through the Medical University of South Carolina EPSCOR training program. My work was done on a pilot project directed at determining the feasibility of using matrix assisted laser desorption ionization time-of-flight mass spectrometry to study post-translational modifications of the visual pigment rhodopsin under physiological conditions. The techniques employed during the course of the project were chemical measurement, mass spectrometry, and signal processing using the MatLab software. In signal processing, techniques such as filtration of data, deconvalution theory, power spectrum analysis, curve fitting, and cross correlation were used. This project was funded through a grant from the National Science Foundation. I would like to give special thanks to Dr. D. Wesley Corson and Specialist Thomas Fleury of M.U.S.C. for providing this opportunity for me and for all of their guidance and support throughout my ten week internship.

College of Charleston Summer Research Project
During the summer, I have been investigating methods to measure oxygen content during cellular photodynamic therapy in vitro. The objective is to monitor oxygen content in sealed dye-sensitized cell suspensions which are illuminated with a doubled YAG laser. In addition to the oxygen content, we are interested in determining the absorbed light dose. Therefore, I have also investigated the photobleaching of merocyanine 540 during standard PDT illumination conditions. I would like to give special thanks to the Physics Department for the financial support and to Dr. Linda Jones of the College of Charleston for her role in my appointment to this research project and for all of the training I received under her expert tutelidge. Without her none of this would have been possible. Thanks Linda!

College of Charleston Senior Research Project
My senior research project will be completed in December 1998. This project is related to my Summer research in that I will be monitoring the oxygen consumption of A549 cells during Photofrin- and MC540-mediated photodynamic therapy. However, I will also be observing the changes in the optical properties of the cells and dye in media throughout the illumination process. A Nd-YAG laser emitting 532nm light will be used to initiate photodynamic damage, and a helium-neon laser emitting 633nm light will be used simultaneously to measure the diffuse transmittance. The diffuse reflectance of each sample will also be measured using an integrating sphere in conjunction with Macintosh Operating Systems and WorkBench software. This project will also be carried out under guidance from Dr. Linda Jones.

John graduated in December 1998.

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